Convenient Kit for Generating Mutations

Johns Hopkins School of Medicine
Pharmacology and Oncology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

New England Biolabs

Product Name:

Q5 site-directed mutagenesis kit

Catalog Number:

E0552S

I use this kit to do quikchange to introduce all kinds of mutation, deletion and insertion to the DNA of interest. It is very convenient and usually works well.

Experimental Design and Results Summary

Application

Cloning

Starting Material

N/A

Tips

Design the primer using NEB website

Results Summary

The cloning procedure is much easier and success rate of cloning is pretty high

DOI or PMID #

N/A

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Summary

The Good

Convenient and fast

The Bad

None

The Bottom Line

This system is very powerful for those who do a lot of cloning to generate mutations. Price is also reasonable.

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