PCR Enzyme Mix for Next Generation Sequencing Sample Prep

Johns Hopkins School of Medicine
Pharmacology and Oncology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

New England Biolabs

Product Name:

NEBNext® Ultra™ II for DNA Library Prep

Catalog Number:

E7103

I use this kit to PCR amplify the DNA which will be used for next generation sequencing. The enzyme is engineered to be highly specific and unbiased.

Experimental Design and Results Summary

Application

Next generation sequencing sample prep

Starting Material

N/A

Tips

Follow the protocol provided by manufacturer

Results Summary

Highly efficient and specific

DOI or PMID #

N/A

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Summary

The Good

Produce reliable and high quality result

The Bad

Expensive

The Bottom Line

This product is the recommended PCR reagent for standard protocol to prepare next generation sequencing samples. Although it's expensive, but it's well worth it since the sequencing will cost much more so you don't want to take the risk to try other cheaper enzymes.

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