Time Course Effect of Chemoattractant on SIRT5 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

SIRT5 Antibody

Catalog Number:

sc-271635

Clone Number:

G-2

SIRT5 is one of the three sirtuins family localized primarily to the mitochondrion. SIRT5 has been found to exhibit enzymatic activities as a deacetylase, desuccinylase, and demalonylase, capable of removing acetyl, succinyl, and malonyl groups from the lysine residues of proteins. Here we checked the time course effect of SIRT5 expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We observed time dependent of SIRT5 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

This antibody is very good for western blot experiment

The Bad

None

The Bottom Line

This antibody is very specific and works great for western bot. Highly recommended.

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