NLRP3 (D4D8T) Rabbit mAb for Total NLRP3 Protein

Pediatrics
Drexel University
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

NLRP3 (D4D8T) Rabbit mAb

Catalog Number:

15101

Browse Similar Products: NLRP3 Western Blot Antibodies

NLRP3 is an important marker for cellular stress signals. I have used to see the changes in our experiment of cell stress.This is a good antibody and highly reactive in both human and mice samples.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mice tissue

Primary Incubation

1:1000, Over night at 4 degree on shaker

Blocking Agent

LICOR blocking buffer

Secondary Incubation

1:5000, 1hr at room temp

Tertiary Incubation

N/A

Detection

LICOR Odyssey imaging system

Results Summary

In our experiments results were very good. Easy to standardize. Used antibody can be reused for many blots if stored properly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to standardize. Used antibody can be reused for many blots if stored properly.

The Bad

None

The Bottom Line

I recommend NLRP3 (D4D8T) Rabbit mAb for endogenous levels of total NLRP3 protein

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