MAB201 (R&D Systems) Binds to Recombinant Human IL-1 Beta

November 01, 2019

BlueskyReddit
Biologics
AbbVie
Research Scientist

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Company:

R&D Systems

Product Name:

Human IL-1 beta /IL-1F2 Antibody

Catalog Number:

MAB201

Clone Number:

8516

MAB201 binds Recombinant Human IL-1 beta (201-LB, R&D Systems) with picomolar affinity.

Experimental Design and Results Summary

Applications

Biacore (Surface Plasmon Resonance)

Sample

Recombinant human IL1b

Primary Incubation

CM5 chip with immobilized goat anti mouse IgG Fc

Blocking Agent

BSA

Secondary Incubation

5min and 50ul/min flow rate

Tertiary Incubation

N/A

Detection

SPR

Results Summary

MAB201 was detected as binding recombinant humanIL1b with an affinity of approximately 7pM as measured by biacore.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Product binds expected antigen.

The Bad

Endotoxin levels in product unknown

The Bottom Line

The antibody MAB201 binds human-IL1b and can be used in other assays.

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