R&D Systems MAB200 Binds Recombinant Human IL1a with Affinity of 200pM

October 29, 2019

Biologics
AbbVie
Research Scientist

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Company:

R&D Systems

Product Name:

Human IL-1 alpha /IL-1F1 Antibody

Catalog Number:

MAB200

Clone Number:

4414

Biacore analysis indicated that MAB200 binds Interleukin-1-alpha (Roche, cat.no. 3501370) with high affinity.

Experimental Design and Results Summary

Applications

Biacore (Surface Plasmon Resonance)

Sample

Recombinant human IL1a

Primary Incubation

CM5 chip with immobilized goat anti mouse IgG Fc

Blocking Agent

BSA

Secondary Incubation

5min and 50ul/min flow rate

Tertiary Incubation

N/A

Detection

SPR

Results Summary

Binding affinity of 200pM was detected for MAB200 to recombinant human IL1a form Roche.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Product binds antigen with high affinity

The Bad

Expensive

The Bottom Line

The antibody can be used in subsequent IL1a binding assays.

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