TLR4/MB2 Does Not Bind Tennacin by SPR

AbbVie
Biologics
Research Scientist

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Company:

R&D Systems

Product Name:

Recombinant Human TLR4/MD2 Complex Protein

Catalog Number:

3146-TM

Binding of TLR4/MD2 complex to tennacin cFBG and c-FL was analyzed by SPR. No binding was observed.

Experimental Design and Results Summary

Application

Biacore (Surface Plasmon Resonance)

Starting Material

Human Tenascin-C

Tips

Binding was not observed by SPR.

Results Summary

TLR4/MD2 (3146-TM) did not bind Tennacin c-FBG or Tennacin c-FL as expected.

DOI or PMID #

N/A

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Summary

The Good

It is a pre made complex ready for use.

The Bad

Binding was not observed to Tennacin.

The Bottom Line

Binding was not observed by SPR, but orthogonal methods may be able to detect binding, such as cellular assays.

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