Basic Buffer for Trypsin Digestion

October 23, 2019

BlueskyReddit
Seoul National University
Biology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

AlfaAesar

Product Name:

EPPS, 0.2M buffer soln., pH 8.5

Catalog Number:

J61476-AE

Convenient pre-made buffer for basic pH

Experimental Design and Results Summary

Application

Basic buffer preparation for protein digestion with trypsin

Starting Material

Human tissue culture cell line HEK293T and HCT116

Tips

Works well

Results Summary

This buffer is mass-spec compatible. Trypsine and LysC digestion of protein samples in this buffer works well.

DOI or PMID #

N/A

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Summary

The Good

Convenient and consistent pH

The Bad

None

The Bottom Line

Good for Mass-Spec sample preparation

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