Efficient DNA Transfection Kit

October 21, 2019

BlueskyReddit
Seoul National University
Biology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fischer Scientific

Product Name:

Lipofectamine® 3000 Transfection Reagent

Catalog Number:

L3000008

I use this for all the lent-viral plasmid transfection.

Experimental Design and Results Summary

Application

DNA transfection

Starting Material

Human tissue culture cell line HEK293T, HEK293 and HCT116

Tips

Use less than required and wash out fast

Results Summary

This regents work very well for DNA transfection. I use this for lentivirus vector mediated protein expression.

DOI or PMID #

N/A

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Summary

The Good

Efficient transfection

The Bad

Quite expensive

The Bottom Line

Good for small scale transfection experiments.

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