Excellent and Specific Ulk1 Antibody

October 24, 2019

BlueskyReddit
cell biology
Harvard Medical School
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

ULK1 (D8H5) Rabbit mAb

Catalog Number:

8054

Browse Similar Products: ULK1 Western Blot Antibodies

I work on various aspects of the autophagy-lysosomal system in Mammalian cells. Specially I am interrogating the substrate repertoire of autophagy using CRISPR-Cas technology as well as quantitative multiplexed proteomics. This Ulk1 antibody was super clean.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Cell Line, Patu-8988T

Primary Incubation

1:1000, Overnight, 4C

Blocking Agent

5% Milk, TBST

Secondary Incubation

1:10,000, anti Rabbit HRP,1hr room temp

Tertiary Incubation

N/A

Detection

ECL (BioRad Chemi Doc)

Results Summary

Super clean Ulk1 antibody. Strong signal which is lost in knockout cell lysate indicating clean specificity.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Clean and specific antibody

The Bad

None

The Bottom Line

Use it! CST succeeds again.

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