Does Not Detect Fam46a

October 23, 2019

Pathology and laboratory medicine
University of Pennsylvania
Graduate Student

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Company:

Biorbyt

Product Name:

FAM46A antibody

Catalog Number:

orb183604

Clone Number:

Polyclonal

Do an IF with HA-tag Fam46a. The signal from this antibody does not co-localize with anti-HA signal.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human, HEK293t

Primary Incubation

1:100

Blocking Agent

BSA

Secondary Incubation

1:500, donkey anti-rabbit alexa flour 488

Tertiary Incubation

No

Detection

Microscopy

Results Summary

Detect HA-tag Fam46a with both anti-HA antibody (Alexa flour 647) and this antibody (Alexa flour 488). The signal from this antibody does not colocalize with anti-HA signal, suggesting this antibody does not specifically detect Fam46a.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Has trial size

The Bad

Does not work

The Bottom Line

This antibody does not detect Fam46a.

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