Time Course Effect of Chemoattractant on TGFß1 Expression

October 24, 2019

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

TGFβ1

Catalog Number:

sc-130348

Clone Number:

3C11

Transforming growth factor βs (TGFβs) has the ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFα. Besides that TGFβs mediate many cell-cell interactions that occur during embryonic development. Three TGFβs have been identified in mammals. TGF β1, TGF β2 and TGF β3. Here we checked the time course effect of of TGFβ on chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

Observed time dependent increase of TGFβ1 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

This antibody is very good for western blot experiment

The Bad

None

The Bottom Line

This antibody is very good for western blot. Highly recommended.

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