eBioscience (Thermo Fisher Scientific - Invitrogen)
CD4 Monoclonal Antibody (RPA-T4), PerCP-Cyanine5.5, eBioscience™
45-0049-42
RPA-T4
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Browse Similar Products: CD4 Flow Cytometry Antibodies
Our research involves the study of immune cell phenotypes in different disease states with a focus on viral infection. This antibody was used to assess the purity of CD4+ T cells that were positively selected from total peripheral blood mononuclear cells.
Flow Cytometry
Purified CD4+ T cells from human PBMC by magnetic bead-based positive selection
2.0x10^5 cells were stained with the manufacturer's recommended volume of antibody in a total staining volume of 100ul autoMACS running buffer (Miltenyi Biotec) for 30 minutes on ice in the dark.
10 min on ice with human Fc receptor blocking reagent (Miltenyi Biotec) prior to primary incubation with fluorochrome-labelled antibody
N/A
Becton Dickinson FACSCalibur flow cytometer
This antibody resulted in a distinct population of CD4+ cells (unfilled histogram) that are easily distinguished from CD4+ cells stained with a matched isotype control antibody (filled histogram). >95% of purified CD4+ T cells stained for CD4.
10.1186/s12865-015-0113-0
Bright staining. Little optimization required other than initial titrating.
None
Recommended for identifying CD4+ cells in PBMC samples or purified T cell preparations by flow cytometry.