PCR Master Mix for Superior Performance

Folium Food Science
Microbial Product Development
Group/Project Leader

Overall

Quality of Results

Ease-of-Optimization

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Company:

NEB Biolab Inc.

Product Name:

Phusion® High-Fidelity PCR Master Mix with HF Buffer

Catalog Number:

M0531S

I have been using this PCR master mix for the last six years, and I always get positive results by using this PCR master mix. Phusion High-Fidelity DNA Polymerase has both high fidelity and robust performance and thus can be used for all PCR applications. This master mix is an ideal choice for cloning and can be used for long or difficult amplicons.

Experimental Design and Results Summary

Application

PCR Amplification

Starting Material

DNA/genomic DNA

Tips

DNA use usually 2 ng to 20 ng

Results Summary

We generally use this PCR master mix for large and complex amplification and always get expected band without any unspecific band.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Specific band

The Bad

Price

The Bottom Line

Highly recommended

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