Good Option to Stain Mouse CD95 (FAS)

October 09, 2019

BlueskyReddit
B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PE Hamster Anti-Mouse CD95

Catalog Number:

554258

Clone Number:

Jo2

Browse Similar Products: CD95 Flow Cytometry Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining germinal center B cells (GL7+CD95+).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

C57Bl/6 spleen

Primary Incubation

Surface staining with CD95 PE (1/200), 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We could detect a population of CD95+GL7+ GC B cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It works

The Bad

Is expensive

The Bottom Line

Ok option to stain mouse CD95 (FAS).

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