Abcam
Anti-alpha tubulin antibody
ab7291
DM1A
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I am a chemical biologist, and my research focuses on identifying small molecules that selectively target cellular processes involved in maintaining protein homeostasis. Therefore, I perform chemical, biochemical, and cell biological assays relevant to protein synthesis (ribosome) and degradation (the ubiquitin proteasome system and autophagy). This antibody worked well even when the membrane was stripped several times.
Western Blot
HEK293T
1:5000 in 5% milk
5% milk, 10 min
Anti-rabbit HRP, 1:5000
N/A
Chemidoc (biorad), ECL
I use this antibody at the end of every WB process to get the loading control blot. Always works well.
Always works
This is a great antibody for loading control.