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PE/Cy7 anti-mouse CD93 Antibody
136505
AA4.1
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining immature and mature B cells.
Flow Cytometry
C57BL/6 spleen and BM
Surface staining with CD93 PE-Cy7 (1/200), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
As the majority of the B cells in the BM are immature they stain positive for CD93, with only a fraction of recirculating mature B cells being negative for CD93. In the spleen, the great majority of the B cells are mature and negative for CD93.
It works well
Could be brighter
Good option to stain murine CD93.