Isolate Plasmid DNA Bacterial Cultures

October 18, 2019

BlueskyReddit
Havard Medical School
Dpt of Cell Bio
Postdoctoral Researcher

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Company:

Omega Bio-tek

Product Name:

E.Z.N.A.® Plasmid Mini Kit I, (Q-spin)

Catalog Number:

D6942-02

This mini-prep kit is used to purify plasmid DNA for various constructs from bacterial culture (5 ml).

Experimental Design and Results Summary

Application

Mini-prep plasmid DNA isolation

Starting Material

5 ml of bacterial cultures grown overnight

Tips

None. Just follow the provided protocol.

Results Summary

We routinely successfully isolate ~20-30 µg of high-quality plasmid DNA with this kit.

DOI or PMID #

10.1016/j.molcel.2019.03.034

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Summary

The Good

Ease of use. High-quality plasmid. Half the price of Qiagen Miniprep Kit

The Bad

None!!

The Bottom Line

This Mini-prep plasmid kit is very easy to use and yield good amount of high-purity DNA plasmid for half the price of Qiagen Miniprep Kit.I highly recommend it to anyone.

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