Excellent TMEM115 Antibody from Atlas Antibodies

Sir William Dunn School of Pathology
Oxford University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Atlas Antibodies

Product Name:

Anti-TMEM115

Catalog Number:

HPA015497

Clone Number:

Polyclonal

I am studying Golgi function and used TMEM115 as a marker protein. The MEF cells were transfected with the negative control, or two independent siRNAs targeting TMEM115 for 72 h. The cells were then lysed, and processed for WB. This antibody could specifically detect endogenous TMEM115 both in IF and WB.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MEF

Primary Incubation

1:1000 in 1% milk in PBST, 4 degree, overnight

Blocking Agent

1% milk in PBST, room temperature for 1 h

Secondary Incubation

1:5000 in 1% milk in PBST, room temperature for 1h

Tertiary Incubation

N/A

Detection

ECL

Results Summary

It detects endogenous TMEM115 specifically at ~ 35 kd.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specifically detects endogenous protein

The Bad

N/A

The Bottom Line

An excellent antibody for studying TMEM115

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