Exceptional Performance with Long Amplicons

Folium Food Science
Microbial Product Development
Group/Project Leader

Overall

Quality of Results

Ease-of-Optimization

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Company:

NEB Biolab Inc.

Product Name:

LongAmp® Taq PCR Kit

Catalog Number:

E5200S

LongAmp Taq DNA Polymerase is a unique Taq DNA Polymerases. Which Optimized enzyme for amplification up to 30 kb from low complexity templates and difficult templates. I used this kit for amplification of up to 15kb and I got one shot result without any optimization. LongAmp Taq DNA Polymerase have two fold higher fidelity than Taq Polymerase alone. The Kit is supplied with LongAmp Taq Reaction Buffer, which is optimized to give the highest PCR yields.

Experimental Design and Results Summary

Application

PCR Amplification

Starting Material

DNA

Tips

Setting for gradient PCR reaction for difficult fragments

Results Summary

We amplify 12kb gene from bacterial genomic DNA and we got exact amplicon after confirming sequencing.

DOI or PMID #

N/A

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The Bottom Line

Highly recommend for PCR amplification of more than 10kb.

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