Plasmid Prepration with Qiagen Kit

Folium Food Science
Microbial Product Development
Group/Project Leader

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

QIAGEN

Product Name:

QIAprep Spin Miniprep Kit

Catalog Number:

27104

This kit is used to purify plasmid from bacteria culture. The kit has simple instructions and produces high quality plasmids with good 260/280 and 260/230 readings. I routinely perform 10-20 miniprep a day. This kit always gives me high yields (500-100 ng DNA per uL) depending upon plasmid copy no. and cell volume.

Experimental Design and Results Summary

Application

Plasmid Purification

Starting Material

Bacterial cell culture with plasmid inside

Tips

Cell should be OD. 600 1-2

Results Summary

The isolated plasmid DNA is high quality with good 260/230 and 260/280 numbers. The plasmid has also been used in subsequent cloning applications. Thus, it is good to use with downstream applications.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy to use

The Bad

Price

The Bottom Line

This is a high-quality and quantity kit for plasmid purification.

Join the discussion