Great CST VDAC Antibody

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Techonolgy

Product Name:

VDAC Rabbit mAb

Catalog Number:

4661

Browse Similar Products: VDAC Western Blot Antibodies

We used the CST rabbit monoclonal antibody against mitochondrial protein VDAC to optimize a mitochondria isolation method. The antibody was proved to be highly specific with low background.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Jurkat

Primary Incubation

1:2000, OVN, 4 degree

Blocking Agent

5% milk, 1hr

Secondary Incubation

1:5000, RT, 1hr

Tertiary Incubation

None

Detection

ECL

Results Summary

A mitochondria isolation kit was used to isolate mitochondria versus cytoplasmic portion from Jurkat T cells. Lysates were probed with VDAC antibody and was shown to localized to mitochondria portion only.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High signal to noise ratio

The Bad

None for western blot

The Bottom Line

Ideal antibody for western blot to look at VDAC.

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