Good Zebrafish Antibody for Nlz2

Biology
University of Kentucky
Principal Investigator

Overall

Quality of Results

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Company:

Boster Bio

Product Name:

Polyclonal anti-nlz2 antibody

Catalog Number:

DZ12892

Clone Number:

Polyclonal

Browse Similar Products: Nlz2 Immunofluorescence Antibodies

A custom generated anti-zebrafish nlz2 antibody that works in whole mount IF applications.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Zebrafish embryos 24hpf

Primary Incubation

1/50, overnight

Blocking Agent

BSA

Secondary Incubation

1/1000, overnight

Tertiary Incubation

None

Detection

Confocal Microscopy

Results Summary

Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Zebrafish-specific antibody

The Bad

The signal is not entirely clean

The Bottom Line

This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise.

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