Good Zebrafish-Specific Siah1 Antibody from Boster Bio

October 15, 2019

BlueskyReddit
Biology
University of Kentucky
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

Boster Bio

Product Name:

Polyclonal anti-Siah1 antibody

Catalog Number:

DZ02095

Clone Number:

Polyclonal

Browse Similar Products: Siah1 Immunofluorescence Antibodies

Custom generated Siah1 antibody against the zebrafish homologue.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Zebrafish embryos 5dpf

Primary Incubation

1/100, overnight

Blocking Agent

BSA

Secondary Incubation

1/1000 overnight

Tertiary Incubation

None

Detection

Confocal Microscopy

Results Summary

Antibody labels the retina outer segments and inner nuclear layers as expected based on in situ hybridization studies. The staining is clean.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Zebrafish specific antibody

The Bad

Does not work well in whole mount applications

The Bottom Line

A zebrafish-specific antibody for Siah1 that actually WORKS!

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