Good CXCR3 Antibody from BioLegend

August 27, 2019

BlueskyReddit
B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421 anti-mouse CD183 (CXCR3) Antibody

Catalog Number:

126522

Clone Number:

CXCR3-173

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining CXCR3 plasma cells (CD138hi).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

C57B/6 spleen

Primary Incubation

Surface staining with CXCR3 BV421 (1/100), 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We could clearly stained CXCR3+ and negative plasma cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Is specific and good

The Bad

N/A

The Bottom Line

Works well

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