Cell Signaling Technology
IKBA
4812
44d4
Browse Similar Products: I-Kappa-B-Alpha Western Blot Antibodies
Used this IKBA antibody for western blotting and worked very well. The antibody was titrated at four different concentration using HepG2 cell lysate at 20, 10, 5 and 2.5 ug/well. The cells were untreated and this experiment was just to validate the antibody and to see the linearity of its specific binding. The photo is attached.
Western Blot
Human cell lysate
1:1000
5% milk
1:40000
Colorimetric detection
Very nice bands were obtained using HepG2 lysate
N/A
Perfect staining and clear bands
A bit pricey
Detected lysate down to 5 ug/well