I Use This Li-Cor Stain Kit for Total Protein Level Quantificiation

Harvard Medical School
Cell Biology
Postdoctoral Researcher

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Quality of Results

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Company:

Li-Cor

Product Name:

REVERT™ Total Protein Stain Kit, 250 mL

Catalog Number:

926-11016

I work on selective forms of autophagy, especially focusing on ribophagy and ER-phagy. I have used large numbers of antibodies for proteins related to autophagy. This staining kit is way better than Ponceau staining!

Experimental Design and Results Summary

Application

Total protein level quantification for WB quantification

Starting Material

N/A

Tips

Incubate longer than 5 min. It works better that way.

Results Summary

This is superior to ponceau staining. Very sensitive and accurate for total protein level quant as a normalization factor for WB target protein level quantification.

DOI or PMID #

N/A

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Summary

The Good

High sensitivity

The Bad

A bit long procedure compare to ponceau staining

The Bottom Line

I highly recommend this product!

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