Not Very Specific FAM134B Antibody

Cell Biology
Harvard Medical School
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech Group

Product Name:

FAM134B

Catalog Number:

21537-1-AP

Clone Number:

Polyclonal

I work on selective forms of autophagy, especially focusing on ribophagy and ER-phagy. I have used large numbers of antibodies for proteins related to autophagy. This FAM134B antibody is not very specific... but it worked okay.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HCT116 lysate

Primary Incubation

1:1000 in 5% BSA

Blocking Agent

5% non-fat milk in TBST

Secondary Incubation

1:20000 IRdye 800

Tertiary Incubation

Washed three times

Detection

Odyssey

Results Summary

This antibody was okay but not the best. There were many non-specific bands.

DOI or PMID #

10.1016/j.molcel.2019.03.034

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Band was detected

The Bad

Lots of other bands were detected too

The Bottom Line

Not the best antibody

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