Lots of Background Bands from This NUFIP1 Antibody

September 25, 2019

Cell Biology
Harvard Medical School
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

GeneTex

Product Name:

NUFIP1

Catalog Number:

GTX118425

Clone Number:

Polyclonal

I work on selective forms of autophagy, especially focusing on ribophagy and ER-phagy. I have used large numbers of antibodies for proteins related to autophagy. This NUFIP1 antibody has low specificity. There are lots of background bands all the time...

Experimental Design and Results Summary

Applications

Western Blot

Sample

HCT116, HEK293T, HEK293 cells

Primary Incubation

1:1000 dilution in 5% BSA

Blocking Agent

5% non-fat milk

Secondary Incubation

1:20000 IRdye800 rabbit

Tertiary Incubation

Washed three times

Detection

OdysseyCLx

Results Summary

This antibody has been KO validated. It was difficult to find KO clones due to lots of non-specific bands above and below the target band. I do not recommend this antibody but there is no good substitute. I tested another NUFIP1 antibody from Protein technology and it was similarly bad.

DOI or PMID #

N/A

Additional Notes

Salirasib can be used to investigate Ras signaling.

Related Categories

Image Gallery

Summary

The Good

At least it shows the right band

The Bad

Lots of background bands

The Bottom Line

Not a great antibody

Share your experience with other scientists. Write a Review! »

Join the discussion