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Brilliant Violet 510 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody
108457
RB6-8C5
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining B cell in spleen and BM. And use anti-GR1 to exclude granulocytes from our analysis.
Flow Cytometry
WT spleen and bone marrow
surface staining with anti-GR1 BV510 (1/1000), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
We could stain granulocytes that are abundant in the BM, but also present in the spleen.
Good and cheap
Very good option to stain GR1