Highly Specific and Sensitive ATG14 Antibody from CST

Cell Biology
Harvard Medical School
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

ATG14

Catalog Number:

5504

I work on selective forms of autophagy, especially focusing on ribophagy and ER-phagy. I have used large numbers of antibodies for proteins related to autophagy. I used this ATG14 antibody to study autophagy and it worked quite well.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HCT116 cell lysate

Primary Incubation

1:1000 dilution in 5% BSA, TBST. overnight incubation at 4 degree

Blocking Agent

5% non-fat milk in TBST

Secondary Incubation

1:20000 IRdye 800 rabbit

Tertiary Incubation

Washed three times, 5 min before detection

Detection

Odyssey clx

Results Summary

This antibody is very specific and sensitive. I recommend this to others who study autophagy.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good sensitivity and specificity

The Bad

N/A

The Bottom Line

This antibody is very good and selective just like many other CST antibodies!

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