Strong Antibody for Autophagy Receptor CALCOCO2

Cell Biology
Harvard Medical School
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Proteintech Group

Product Name:

CALCOCO2

Catalog Number:

12229-1-AP

Clone Number:

Polyclonal

I work on selective forms of autophagy, especially focusing on ribophagy and ER-phagy. I have used large numbers of antibodies for proteins related to autophagy. This CALCOCO2 antibody worked very well even with short incubation time.

Experimental Design and Results Summary

Applications

Western Blot

Sample

293T cell lysate

Primary Incubation

1:2000 dilution in 5% BSA, TBST. 1h at r.t.

Blocking Agent

5% non-fat milk in TBST

Secondary Incubation

1:20000 dilution of Licor IRdye 800 rabbit

Tertiary Incubation

Washed with TBST three times for 5 min each.

Detection

Odyssey Clx

Results Summary

I used this antibody to see the CALCOCO2 reduction after starvation in the total cell lysate. This experiment worked beautifully. Very sensitive antibody and little background proteins.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Sensitive antibody

The Bad

Nothing

The Bottom Line

This antibody is good to study calcoco2 as a selective autophagy receptor.

Share your experience with other scientists. Write a Review! »

Join the discussion