Very Strong Signal for Phospho-S6 Ribosomal Protein!

September 09, 2019

Cell Biology
Harvard Medical School
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

phospho-S6 ribosomal protein Ser253, 236

Catalog Number:

4858

I work on selective forms of autophagy, especially focusing on ribophagy and ER-phagy. I have used large numbers of antibodies for proteins related to autophagy. This Phospho-S6 Ribosomal Protein antibody is really good. One of the best.

Experimental Design and Results Summary

Applications

Western Blot

Sample

293T, HCT116 cells

Primary Incubation

1:2000 dilution in 5% BSA, TBST. overnight at 4C

Blocking Agent

5% non-fat milk in TBST

Secondary Incubation

1:20000 Odyssey IRDye800 (rabbit)

Tertiary Incubation

Wash TBST, 5min, 3 times

Detection

Odyssey Clx

Results Summary

This antibody is very strong and specific. There is a thin band (off target) above the pS6, but overall specificity is very high.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very good.

The Bad

N/A

The Bottom Line

This antibody is one of the best antibodies I have ever used.

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