Antibody Works Well for Keima Processing Assay

September 06, 2019

BlueskyReddit
Cell Biology
Harvard Medical School
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

MBL international

Product Name:

Keima antibody

Catalog Number:

M182-3M

Clone Number:

1C3

I work on selective forms of autophagy, especially focusing on ribophagy and ER-phagy. I have used large numbers of antibodies for proteins related to autophagy. This Keima antibody works very well to do quantitative WB.

Experimental Design and Results Summary

Applications

Western Blot

Sample

293T and HCT116, HEK293 cells

Primary Incubation

1:2000, overnight incubation

Blocking Agent

5% non-fat milk in TBST

Secondary Incubation

1:20000 IRdye700-mouse

Tertiary Incubation

Wash with TBST three times

Detection

Odyssey clx

Results Summary

This antibody is quite selective for Keima protein. Not many back-ground bands were detected.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Intensity of the band

The Bad

Nothing

The Bottom Line

I highly recommend using this antibody for Keima processing assay by WB.

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