BD Biosciences
PE-Cy7 Hamster Anti-Mouse CD95
557653
Jo2
Browse Similar Products: CD95 Flow Cytometry Antibodies
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining germinal center B cells.
Flow Cytometry
C57BL/6 splenocytes
Surface staining with anti-CD95 PE-Cy7 (1/200) and GL7, 20 min at 4C
Fc block (2.4G2 clone)
N/A
LSR Fortessa
Germinal center B cells are GL7+CD95+.
It works well
Some background staining
Ok to use