Sigma-Aldrich
Propidium Iodide dye
P4170
To study the cell cycle, we have stained the control and treated human neuroblastoma cell lines with propidium iodide dye. After completion of toxin treatment cells were trypsinized, washed twice with PBS and fixed in 70% ice cold ethanol for 24 h at -20 degree C. After completion of cell fixation in ethanol, cells were washed twice with PBS and incubated with PI staining buffer containing propidium iodide, RNAse, 0.05% tritonX-100 in PBS for 30 minutes in dark. After that cells were analysed by flow cytometer in FL3 channel.
Flow cytometry
IMR32 cell line
Prepare 1mg/ml stock solution of propidium iodide in RNAse free water.
IMR32 cells in different phases of cell cycle was recorded in FL3 channel of the flow cytometer.
N/A
Economical and easy to use
None
Before satining cells with propidium iodide, fixed cells in ice cold ethanol always and also add RNAse to the staining buffer to get better results.