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PE/Cy7 anti-mouse CD24 Antibody
101822
M1/69
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. In this experiment, we aimed at characterizing several B cell subsets. CD24 is expressed by B cells but with different intensities depending on the stage of maturity.
Flow Cytometry
C57BL/6 spleen
Surface staining with anti-CD24PE-Cy7 (1/1000), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
B cells (CD19+) express CD24. Several expression levels of CD24 can be distinguished.
Works very well at 1/1000
Is a good and cheap option to stain mouse CD24