Good Option to Stain Mouse CD21/35 (BioLegend)

September 02, 2019

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP/Cy5.5 anti-mouse CD21/CD35 (CR2/CR1) Antibody

Catalog Number:

123416

Clone Number:

7E9

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining B cells subsets (CD19+) in the spleen of WT C57BL/6.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Spleenocytes WT C57BL/6

Primary Incubation

Surface staining with anti-CD21/35 (1/100), 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We could stain the Marginal zone (CD21/35hiCD23lo) and Follicular B cells (CD21/35loCD23hi) within CD19+ cells.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

It works

The Bad

Could be cheaper

The Bottom Line

Ok option to stain mouse CD21/35

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