Thermo Fisher Scientific
FOXP3 Monoclonal Antibody (FJK-16s), PerCP-Cyanine5.5, eBioscience™
45-5773-82
FJK-16s
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MC38 tumors were excised from B6 mice and digested with Collagenase IV (1 mg/ml) and DNaseI (200 ug/ml). Cells were then stained with L/D Blue for gating out dead cells. Subsequently, cells were stained with a range of different antibodies to identify a range of immune cell types. Foxp3- percpcy5.5 from Thermo was one of the antibodies used at a 1:200 dilution. Antibodies were incubated with the tumor cell suspension for 30 minutes at 4 deg C.
Flow Cytometry
Mouse Tumor
1:200
Fc block
N/A
Flow Cytometry (BD Fortessa)
Foxp3+ cells could be clearly gated after tumor cell suspension was stained with antibodies to identify immune cells. First, cells were gated on CD45, then on TCRb, then on CD4 and then output for Foxp3- percpcy5.5 (1:200).
Stains quite bright
Good antibody for staining mouse Foxp3+ cells.