BD Biosciences
CD5 Rat anti-Mouse, Brilliant Violet 786
740842
53-7.3
Browse Similar Products: CD5 Flow Cytometry Antibodies
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. In this experiment, we aimed at quantifying B-1a B cells (CD5+) in the peritoneal cavity of B6 mice.
Flow Cytometry
Peritoneal Cavity lavage from WT B6 mice
surface staining with CD5 BV786, 20 min at 4C, 1:100
Fc block (2.4G2 clone)
N/A
Flow cytometer with a violet laser
We could detect B1a (CD5+) cells, as well as T cells that are CD5++
Detects T and B cells
Good option for CD5