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PE-Dazzle 594 anti-mouse/human CD11b Antibody
101255
M1/70
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining B cell subsets (CD19+) in the peritoneal cavity of C57B/6 mice. B1 cells that are enriched in the peritoneal cavity express intermediary amounts of CD11b, while macrophages express high amounts of CD11b.
Flow Cytometry
Peritoneal cavity of C57Bl/6
Surface staining with anti-CD11b, 20 min at 4C dil: 1/4000
Fc block (2.4G2 clone)
N/A
Flow cytometer
B1 B cells that are enriched in the peritoneal cavity express intermediary amounts of CD11b, while macrophages express high amounts of CD11b.
Stains macrophages and B Cells
Is used at 1/4000 so it is very inexpensive.