Isolate RNA with Direct-zol RNA MiniPrep

August 15, 2019

BlueskyReddit
UAB
Radiation Oncology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Zymo Research

Product Name:

Direct-zol RNA MiniPrep

Catalog Number:

R2051

RNA was easily extracted from human breast cancer cells with the Direct-zol RNA MiniPrep prior quantification and running RT-PCR.

Experimental Design and Results Summary

Application

PCR

Starting Material

Human breast cancer cells

Tips

Make sure to read the complete instructions provided online prior to using the kit in order to be aware of the details.

Results Summary

According to the instructions provided, less than 5 million cells yielded a considerable of RNA of good quality (according to A260/280 value by Nanodrop), sufficient for PCR.

DOI or PMID #

N/A

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Summary

The Good

Quick and simple to use

The Bad

When capped, tubes do not fit exactly 1200 uL of liquid following addition of ethanol

The Bottom Line

The Zymo RNA MiniPrep kit enables a researcher to extract much high-quality RNA very quickly which is ideal for many applications such as PCR.

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