Great Vascular Endothelial Cell Marker from Novus

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Von Willebrand Factor Antibody

Catalog Number:

NB600-586

Clone Number:

Polyclonal

The aim of our research is to study vascular abnormalities in neurodegenerative diseases. The specific goal of this experiment was to quantify the number of vessels in tissue specific regions. This antibody demonstrated excellent staining for vascular endothelial cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Feline eye

Primary Incubation

Diluted at 1:1000, overnight at 4°C

Blocking Agent

10% normal donkey serum + 0.5% triton X + 0.1M PBS

Secondary Incubation

Alexa 488 conjugated anti-mouse IgG 1:500, 1 hr at RT

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Robust staining for vascular endothelial cells (red), with no unspecific binding or background in the feline sclera.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Highly specific

The Bad

N/A

The Bottom Line

This antibody worked well as vascular endothelial markers in cats.

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