Keratin 5 Polyclonal Antibody, Purified
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We study epithelial-immune cell interactions in healthy and PyMT transformed mammary gland. In order to detect K5+ epithelial cells, we tested this polyclonal antibody with excellent results.
PLP fixed mouse (C57BL/6) salivary gland cryosections
1 in 200 for 3 hr at room temperature
1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum, 2% goat serum
Goat anti-rabbit Alexa Fluor 647 (1:500) 1hr at room temperature
Images were acquired using an inverted Zeiss LSM880 confocal microscope with an 20x 0.8NA air objective.
K5 (red), E-cadherin (green) & Hoechst (blue)
Reliable & reproducible staining
Very good anti-K5 antibody