BioLegend K14 Antibody for Immunofluorescence

September 23, 2019

Immunology
MSKCC
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Keratin 14 Polyclonal Antibody, Purified

Catalog Number:

905301

Clone Number:

N/A

We study epithelial-immune cell interactions in healthy and PyMT transformed mammary gland. In order to detect K14+ epithelial cells, we tested this polyclonal antibody with excellent results.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PLP fixed mouse (C57BL/6) salivary gland cryosections

Primary Incubation

1 in 200 for 3 hr at room temperature

Blocking Agent

1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum, 2% goat serum

Secondary Incubation

Goat anti-rabbit Alexa Fluor 647 (1:500) 1hr at room temperature

Tertiary Incubation

N/A

Detection

Images were acquired using an inverted Zeiss LSM880 confocal microscope with an 20x 0.8NA air objective.

Results Summary

Excellent staining of K14+ cells: E-cadherin (green), K14 (red), Hoechst (blue)

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very nice staining

The Bad

None

The Bottom Line

Excellent anti-K14 antibody.

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