R&D CD69 Antibody for Immunofluorescence

September 06, 2019

BlueskyReddit
Immunology
MSKCC
Research Fellow

Overall

Quality of Results

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Company:

R&D Systems

Product Name:

Mouse CD69 Antibody

Catalog Number:

AF2386

Clone Number:

N/A

We study tumorigenesis in the PyMT-driven mammary gland tumor mouse model. To visualize lymphocytes in tumors, we tested this polyclonal goat a-CD69 antibody in tumors, with excellent results.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PLP fixed mouse (C57BL/6) PyMT tumor cryosections

Primary Incubation

1 in 100 for 3 hr at room temperature

Blocking Agent

1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum, 2% donkey serum

Secondary Incubation

Donkey anti-goat Alexa Fluor 647 (1:500) for 1 hr at room temperature

Tertiary Incubation

N/A

Detection

Images were acquired using an inverted Zeiss LSM880 confocal microscope with an 20x 0.8NA air objective.

Results Summary

Clear staining of CD69+ cells: CD69 (red), CD3 (green), Hoechst (blue)

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable & reproducible staining

The Bad

None

The Bottom Line

Excellent goat polyclonal anti-CD69 antibody for IF.

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