Time Course Effect of Chemoattractant on Trx Expression

August 14, 2019

BlueskyReddit
Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

Thioredoxin (Trx)

Catalog Number:

sc-166393

Clone Number:

A-5

Trx is a 12 kda multifunctional protein. It is a unique antioxidant protein with various cellular functions. It promotes the cell growth and protect the cells from apoptosis. Here we checked the time course effect of Trx by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found a time-dependent increase of Trx expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody is very good for western blot experiment

The Bad

None

The Bottom Line

This antibody is very specific and the signal is great. Highly recommended.

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