Not Very Specific Doublecortin Antibody

Johns Hopkins University
Postdoctoral Researcher

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Company:

Cell Signaling Technology

Product Name:

Doublecortin Antibody

Catalog Number:

4604

Browse Similar Products: Doublecortin Western Blot Antibodies

My research focuses on understanding how neurons generate their complex morphology and form proper circuitries during development, and how neurons regenerate to restore connections after brain or spinal cord injuries. In this western blot experiment, I want to detect the expression of Doublecortin in mouse retina.

Experimental Design and Results Summary

Applications

Western Blot

Sample

mouse retina

Primary Incubation

1:1000, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:2000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

I ran two similar pairs of protein samples, but the bands look quite different (one pair has one band just as the official website picture shows, however the other one pair has two bands).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

N/A

The Bad

This antibody is not specific

The Bottom Line

I don't recommend this antibody.

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