Very Good P-FAK Antibody from CST

Johns Hopkins University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-FAK (Tyr397) Antibody

Catalog Number:

3283

Browse Similar Products: P-FAK Western Blot Antibodies

My research focuses on understanding how neurons generate their complex morphology and form proper circuitries during development, and how neurons regenerate to restore connections after brain or spinal cord injuries. In this western blot experiment, I want to detect the expression of Phospho-FAK (Tyr397) in mouse CAD cell line.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse CAD cells

Primary Incubation

1:1000, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:2000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

This antibody is quite specific and the band looks quite nice. The differences between the control group and the phosphorylation group can be easily seen.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody works very well

The Bad

N/A

The Bottom Line

I definitely recommend this antibody.

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