Good LIN28B Antibody from CST

Johns Hopkins University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

LIN28B Antibody

Catalog Number:

4196

Browse Similar Products: CD3 Western Blot Antibodies

My research focuses on understanding how neurons generate their complex morphology and form proper circuitries during development, and how neurons regenerate to restore connections after brain or spinal cord injuries. In this experiment, I want to detect the expression of Lin28B in mouse CAD cell line.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse CAD cells

Primary Incubation

1:1000, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:2000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

The antibody is specific; however, the band doesn't look quite pretty.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody is ok.

The Bad

N/A

The Bottom Line

I recommend this antibody.

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